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将相序表的ABC三相接线分别与待测量的三相分别连接，相序表上的灯亮顺序为顺时针转动则为正相序，反之则为反相序。
一般在正式电气线路的接线中，我们可从线的颜色分辨在相关规定中制定，母线的相序颜色涂色是：Ａ相黄色，Ｂ相绿色，Ｃ相红色，PE黑色，PEN黄绿交替色，正极－褚色，负极－兰色
A相为黄色；B相为绿色；C相为红色。
电力线路中A相、B相、C相其实都是人为规定的，在相量中互相差120度相位角。
在必须区分ABC三相的场合，一般有这样几种方法：
一、在电力系统中，来电的相位是标定的，即告知你哪根线是A，哪根线是B，哪根线是C，按照相位色标志是：A相为黄色；B相为绿色；C相为红色。或者直接标明ABC相。
两个相同电压等级、相同频率，但来自不同电源的系统并列时，用等电位法：测量两端电源之间的电位差，用电压表（或电压互感器）两端分别接两个电源的A相，在测量两个B相、C相的电压，如果这三个电压都为零，则说明这两个电源的A、B、C相序相同。可以并列运行。
二、在用户端，使用用电器时，不一定要校准ABC相位，因为，除了电动机等由于旋转方向受相序的影响外，其他用电器对相序没有要求。当然，严格地说，准确标明是可以做到的，就是在施工时用万用表对来电进行校核相位，系统全停电时也可以将进行这项工作，前提是系统相位已经标明。
三相电动势达到最大值（振幅）的先后次序叫作相序。
e1比e2超前120°，e2比e3超前120°，而e3又比e1超前120°，称这种相序为正相序或顺相序；反之，如果e1比e3超前120°，e3比e2超前120°，e2比e1超前120°，称这种相序为负相序或逆相序。
相序是一个十分重要的概念，为使电力系统能够安全可靠地运行，通常统一规定技术标准，一般在配电盘上用黄色标出U相，用绿色标出V相，红色标出W相。
交流电力系统中有三根导线，分为ABC三相，正常情况下三相电压、电流对称，相位相差120°。但在系统出现故障时，ABC三相不再对称，为便于分析，可将电压、电流分解为正序、负序和零序三种分量。
相序可用相序器来测量。
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